R. Bras. Zootec.15/Jul/2019;48:e20180180.

Effects of melanocortin 1 receptor (MC1R) gene polymorphisms on plumage color in mule ducks

Yi-Chen Tu ORCID logo , Liang-Yuan Wei, Yi-Ying Chang ORCID logo , Hsiu-Chou Liu, Hsien-Hsiung Lee ORCID logo , Yu-Hsiang Yu ORCID logo , Ming-Cheng Chen ORCID logo

DOI: 10.1590/rbz4820180180

ABSTRACT

The objective of the present study was to investigate the effect of single nucleotide polymorphism (SNP) of the melanocortin 1 receptor (MC1R) gene on plumage coloration in mule ducks. PCR-high-resolution melting analysis (PCR-HRM) and DNA sequencing were used to identify the SNP variability of the MC1R gene in white common ducks. Three non-synonymous SNP (MC1R gene exon 1, c.52G>A, c.376G>A, and c.409G>A) were identified in white Tsaiya ducks. Mating test (white Tsaiya ducks × white Muscovy drakes) in combination with polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) was performed to investigate the effect of non-synonymous SNP of different maternal lines on plumage coloration in mule ducks. Genotyping results from 58 white Tsaiya ducks revealed the significant associations between genetic variations (c.52G>A, c.376A>G, and c.409G>A) and plumage color in two maternal populations. After genotyping of 266 mule ducks, these three non-synonymous SNP identified in white Tsaiya ducks were significantly associated with plumage color of mule ducks. Therefore, the polymorphisms of MC1R gene at c.52G>A, c.376A>G, and c.409G>A in white Tsaiya duck could be used in marker-assisted selection to improve the plumage color of mule ducks.

Effects of melanocortin 1 receptor (MC1R) gene polymorphisms on plumage color in mule ducks

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