R. Bras. Zootec.. 01/Mar/2017;46(3):185-91.

Determination of the mode of reproduction of bahiagrass hybrids using cytoembryological analysis and molecular markers

Roberto Luis Weiler, Miguel Dall’Agnol, Carine Simioni, Karine Cristina Krycki, Nair Dahmer, Divanilde Guerra

DOI: 10.1590/S1806-92902017000300002

ABSTRACT

The aim of this study was to determine the mode of reproduction of a hybrid progeny derived from intraspecific crosses of Paspalum notatum through cytoembryological analysis and use of RAPD (random amplification of polymorphic DNA) molecular markers. Cytoembryological analysis allowed identification of the mode of reproduction of 28 plants that were selected after agronomic productivity evaluations. Of these, 19 had embryo sac morphology compatible with an apomictic mode of reproduction and nine had embryo sac morphology compatible with a sexual mode of reproduction. Meanwhile, molecular marker analysis for 194 individuals showed 54 sexual and 140 apomictic plants; of the 28 plants analyzed by the two methods, ten results (35.7%) were in disagreement. In this paper, through cytoembryological analyses, a ratio of 1:2.1 of sexual to apomictic plants was found. The BCU 243 marker showed a stable pattern of amplification, but some results differed with cytoembryological analyses, demonstrating that these analyses are more reliable when determining the mode of sexual reproduction for the plants of P. notatum. Apomictic plants characterized in this work can be tested in the field to check their agronomic value and registration as plant varieties, while the sexual plants can be used as potential parents in future crosses.

Determination of the mode of reproduction of bahiagrass hybrids using cytoembryological analysis and molecular markers

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